Table 5A.
Summary of differentially expressed MEP pathway genes identified through microarray analysis in Desiree plants grown at two different temperature regimes.
| Gene | Microarray ID | Transcript number | Chr. | 22/16°C norm exp. n = 16 | SE | 30/20°C norm exp. n = 16 | SE | % difference 30/20 vs. 22/16°C | P-Value |
|---|---|---|---|---|---|---|---|---|---|
| 1-deoxy-D-xylulose-5-phosphate synthase 1 | CUST_33054_PI426222305 | PGSC0003DMT400058821 | 1 | 0.85 | 0.10 | 1.64 | 0.27 | 94.11 | ** |
| 1-deoxy-D-xylulose-5-phosphate synthase 2 | CUST_26081_PI426222305 | PGSC0003DMT400041591 | 11 | 0.90 | 0.11 | 1.87 | 0.26 | 107.91 | *** |
| 1-deoxy-D-xylulose 5-phosphate reductoisomerase | CUST_12968_PI426222305 | PGSC0003DMT400062966 | 3 | 0.89 | 0.04 | 1.18 | 0.12 | 32.29 | * |
| 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase | CUST_4428_PI426222305 | PGSC0003DMT400020794 | 11 | 0.76 | 0.06 | 1.13 | 0.07 | 49.99 | *** |
| Geranylgeranyl pyrophosphate synthase 2 | CUST_50887_PI426222305 | PGSC0003DMT400071599 | 4 | 0.94 | 0.10 | 1.51 | 0.21 | 59.85 | *** |
| 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | CUST_42882_PI426222305 | PGSC0003DMT400058463 | 1 | 1.15 | 0.11 | 0.72 | 0.10 | −36.95 | * |
| 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase | CUST_10751_PI426222305 | PGSC0003DMT400031768 | 8 | 1.51 | 0.15 | 0.78 | 0.05 | −48.36 | *** |
| Isopentenyl-diphosphate Delta-isomerase | CUST_14186_PI426222305 | PGSC0003DMT400060039 | 5 | 1.27 | 0.13 | 0.76 | 0.08 | −40.55 | ** |
| Geranylgeranyl pyrophosphate synthase 4 | CUST_5859_PI426222305 | PGSC0003DMT400006940 | 9 | 1.27 | 0.07 | 0.83 | 0.06 | −34.50 | *** |
The data were generated by Hancock et al. (2014) and the values shown are the mean normalized expression values of 16 replicates ± standard error. Comparison of the 22/16° and 30/20°C temperature regimes is shown as a percentage difference. Statistical analysis was performed using Student t-test and significance of differences are indicated (
P < 0.05,
P < 0.01,
P < 0.001).
Abbreviations: Chr, chromosome.