Table 5C.
Summary of differentially expressed carotenoid pathway genes identified through microarray analysis in Desiree plants grown at two different temperature regimes.
Gene | Microarray ID | Transcript number | Chr. | 22/16°C norm exp. n = 16 | SE | 30/20°C norm exp. n = 16 | SE | % increase 30/20 vs. 22/16°C | P-Value |
---|---|---|---|---|---|---|---|---|---|
Beta-carotene hydroxylase 2 | CUST_42340_PI426222305 | PGSC0003DMT400024575 | 3 | 0.92 | 0.19 | 1.64 | 0.16 | 78.25 | ** |
Beta-carotene hydroxylase 1 | CUST_38469_PI426222305 | PGSC0003DMT400074346 | 6 | 1.10 | 0.08 | 1.52 | 0.16 | 38.08 | * |
Lycopene beta cyclase | CUST_45281_PI426222305 | PGSC0003DMT400027593 | 4 | 0.95 | 0.06 | 1.24 | 0.08 | 30.07 | *** |
Phytoene dehydrogenase | CUST_9305_PI426222305 | PGSC0003DMT400023665 | 3 | 0.85 | 0.05 | 1.21 | 0.11 | 42.32 | ** |
Phytoene synthase 2 | CUST_18568_PI426222305 | PGSC0003DMT400043103 | 3 | 0.77 | 0.10 | 1.39 | 0.44 | 80.28 | *** |
Violaxanthin de-epoxidase | CUST_4692_PI426222305 | PGSC0003DMT400027760 | 4 | 0.82 | 0.08 | 1.17 | 0.10 | 41.63 | *** |
Violaxanthin de-epoxidase related | CUST_50238_PI426222305 | PGSC0003DMT400080158 | 4 | 1.41 | 0.07 | 0.75 | 0.10 | −47.08 | *** |
The data were generated by Hancock et al. (2014) and the values shown are the mean normalized expression values of 16 replicates ± standard error. Comparison of the 22/16° and 30/20°C temperature regimes is shown as a percentage difference. Statistical analysis was performed using Student t-test and significance of differences are indicated (
P < 0.05,
P < 0.01,
P < 0.001).
Abbreviations: Chr, chromosome.