Table 2.
Proteins affected by YycF induction, as detected by proteomic analysis.
| Spot | Ratio of induction1 |
pI | MW (kDa) | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
TIGR4 yycG::kan[pPL100] TIGR4[pLS1RGFP] |
TIGR4 yycG::kan[pPL100] TIGR4[pPL101] |
TIGR4[pPL101] TIGR4[pPLS1RGFP] |
|||||||
| 10 min | 30 min | 10 min | 30 min | 10 min | 30 min | Long-term | |||
| FabK | 2.2 | 2.2 | 2.1 | 2.2 | 1.1 | 1.0 | 1.1 | 5.12 | 34.156 |
| FabD | 2.0 | 2.8 | 1.8 | 2.6 | 1.1 | 1.1 | 1.0 | 4.45 | 33.184 |
| FabG | 2.5 | 2.8 | 2.2 | 2.6 | 1.1 | 1.1 | 1.0 | 5.34 | 25.739 |
| FabF2 | 2.0 | 2.3 | 1.8 | 2.3 | 1.1 | 1.0 | 1.0 | 5.45 | 44.075 |
| FabF12 | 3.5 | 2.8 | 2.6 | 2.6 | 1.4 | 1.1 | 0.7 | 5.29 | 45.157 |
| FabF22 | 1.6 | 2.7 | 1.5 | 2.2 | 1.1 | 1.3 | 1.6 | 5.15 | 42.875 |
| FabF32 | 2.0 | 2.4 | 2.0 | 1.8 | 1.0 | 1.3 | 1.3 | 5.32 | 26.699 |
| AccB | 1.9 | 2.0 | 1.7 | 2.0 | 1.1 | 1.0 | 1.2 | 4.17 | 17.023 |
| AccC | 1.9 | 2.6 | 2.5 | 2.2 | 0.8 | 1.1 | 1.2 | 4.85 | 49.793 |
| AccA | 3.0 | 2.1 | 2.3 | 2.4 | 1.3 | 0.9 | 0.9 | 6.31 | 28.230 |
| YycF3 | >164 | >20.6 | 0.9 | 0.8 | >182 | >25.2 | >32.7 | 4.78 | 24.265 |
| YycF3 | >454 | >68.4 | 1.1 | 0.8 | >413 | >58.6 | >74.8 | 4.71 | 23.251 |
AccA, acetyl-CoA carboxylase carboxyl transferase alpha subunit; AccB, acetyl-CoA carboxylase biotin carboxyl carrier protein; AccC, acetyl-CoA carboxylase biotin carboxylase; FabD, Malonyl CoA-acyl carrier protein transacylase; FabF, Oxoacyl-(acyl-carrier-protein) synthase II; FabG, 3-oxoacyl-(acyl-carrier-protein) reductase; FabK, Enoyl-(acyl-carrier-protein) reductase.
1The ratios were calculated by dividing the mean values of the spot intensities (from three independent experiments) obtained for TIGR4 yycG::kan[pPL100], TIGR4[pPL101] and TIGR4[pPL100] and TIGR4[pLS1RGFP]. The mean values and the standard deviation of the spot intensities are depicted in Supplementary Table S1.
2Different isoforms of FabF.
3Different isoforms of YycF.