Figure 1.

Effects of alcohol on human iPSC-derived hepatic progenitor formation. (A) Diagram of hepatic progenitor differentiation from human iPSCs, and alcohol treatment (day 5-10). (B) Expression levels of hepatic progenitor makers such as AFP, CD133, CK19, and EpCAM were examined by Real-time PCR at day 10 after 5 days of alcohol treatment. (C) Immunostaining of AFP at day 10 in control and alcohol treated groups and the percentage of AFP positive cells. (D) Annexin V positive apoptotic cells were analyzed by flow cytometry at day 10 after alcohol treatment. (E) Representative images of Ki67 staining of hepatic progenitor stage cells derived from human iPSCs and the percent of Ki67 positive cells. *:p<0.05, Scale Bar,100µm.