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. 2016 Jul 18;12(9):1052–1062. doi: 10.7150/ijbs.15811

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Effects of alcohol on human iPSC-derived mature stage hepatocytes. (A) Diagram of mature stage hepatocyte-like cell differentiation and alcohol treatment (day 20-25). (B, C) Immunostaining of ALB and Ki67 (B), and AFP and Ki67 (C) at day 25 mature hepatic cells with or without alcohol treatment. (D) The percentages of ALB, AFP or Ki67 positive cells expressed as the mean of three independent experiments. Expressions of hepatic progenitor markers, AFP, CD133, CK19 and EpCAM examined by Real-time PCR (E) and Alcohol metabolic activities measured (F) at day 25 mature stage hepatocytes. *:p<0.05, Scale Bar, 100µm.