Figure 2.
Inhibition of both HBV antigen expression and HBV replication by gRNA-S4 in HepG2.A64 cells. (A, B) Inhibition of HBsAg and HBV DNA in cell culture supernatant at the indicated times points after transfection of HepG2.A64 cells with gRNA-S4. (C) CCK-8 assay performed at the indicated time points after transfection (absorbance at 450 nm, A450); cell viability did not significantly differ between the gRNA-empty and gRNA-S4 samples on 3 successive days after transfection. (D) qPCR-based determination of concentrations of HBV DNA inside viral nucleocapsids on the 9th day after transfection. (E) Representative views of target S4 region deletion in gRNA-S4-treated cells. Black bars represent deletions. Arrowhead indicates the predicated cleavage site of Cas9. (F) The percentage of each indel type which calculated by the length of indels modulo 3.