Table 3.

Rank of the candidate housekeeping genes of T. urticae under different experimental conditions.

Experimental condition	Rank	RefFinder		ΔCt		geNorm		NormFinder		BestKeeper
		Gene	GMa)	Gene	SDb)	Gene	Mc)	Gene	SVd)	Gene	re)
Acaricide resistance selection	1	CycA	1.86	CycA	0.27	Rp49/Rpl18	0.20	CycA	0.10	GAPDH	0.911
	2	Rp49	2.56	GAPDH	0.28	-	-	GAPDH	0.15	CycA	0.894
	3	GAPDH	2.63	Rp49	0.29	GAPDH	0.21	Rp49	0.19	Rp49	0.858
	4	Rpl18	3.13	Rpl18	0.32	CycA	0.22	Rpl18	0.24	Actin	0.828
	5	TUB	3.34	TUB	0.34	TUB	0.27	TUB	0.26	Rpl18	0.790
	6	Ef1α	5.12	Actin	0.37	Actin	0.30	Actin	0.31	TUB	0.504
	7	Actin	6.24	Ef1α	0.37	Ef1α	0.32	Ef1α	0.31	Ef1α	0.332
Host plant shift	1	GAPDH	2.00	GAPDH	0.82	Rp49/Rpl18	0.10	GAPDH	0.20	GAPDH	0.989
	2	Rp49	2.06	CycA	0.87	-	-	CycA	0.44	Ef1α	0.980
	3	Rpl18	2.63	Rp49	0.87	CycA	0.34	Rp49	0.62	CycA	0.973
	4	CycA	2.78	Rpl18	0.88	GAPDH	0.42	Rpl18	0.65	TUB	0.963
	5	Actin	4.30	TUB	1.16	TUB	0.75	TUB	0.93	Rp49	0.953
	6	TUB	5.23	Ef1α	1.26	Ef1α	0.90	Ef1α	1.08	Rpl18	0.949
	7	Ef1α	6.24	Actin	1.36	Actin	1.03	Actin	1.16	Actin	0.838
Both conditions	1	GAPDH	2.11	GAPDH	0.61	Rp49/Rpl18	0.21	GAPDH	0.22	GAPDH	0.987
	2	Rp49	2.28	CycA	0.62	-	-	CycA	0.29	CycA	0.984
	3	Rpl18	2.38	Rp49	0.64	CycA	0.31	Rp49	0.42	Ef1α	0.979
	4	CycA	2.63	Rpl18	0.67	GAPDH	0.35	Rpl18	0.47	Rp49	0.973
	5	Actin	4.30	TUB	0.82	TUB	0.56	TUB	0.63	Rpl18	0.969
	6	TUB	5.23	Ef1α	0.92	Ef1α	0.67	Ef1α	0.79	TUB	0.967
	7	Ef1α	6.24	Actin	1.00	Actin	0.76	Actin	0.86	Actin	0.901

a)GM: Geometric Mean; b) SD: Standard Deviation of comparative ΔCt; c) M: Average of expression stability values; d) SV: Stability Value; e) r: Pearson's correlation coefficient.