Table 1. Glucosinolate (GSL in μmoles g−1 DW), total phenol content (TPC in GAE 100 mg−1 tissue) and mycelial growth (cm) of S. sclerotiorum in methanolic extracts of ALK-RNAi lines.
Line | IBE | GRA | SIN | ALY | GNA | GBN | Indolic GSL | Total GSL | TPC | Mycelial Diameter |
---|---|---|---|---|---|---|---|---|---|---|
Mock | — | — | — | — | — | — | — | — | — | 9.00 ± 0.24 |
Control | 0.06 ± 0.02 | 0.07 ± 0.05 | 8.67 ± 0.18 | 0.10 ± 0.01 | 57.12 ± 1.82 | 3.47 ± 0.02 | 1.02 ± 0.31 | 70.76 ± 2.11 | 0.93 ± 0.09 | 6.32 ± 0.25a |
ALK-RNAi#1 | 0.44 ± 0.02 | 6.27 ± 0.43* | 3.06 ± 0.09 | 1.62 ± 0.09 | 16.02 ± 1.64 | 0.17 ± 0.10 | 0.47 ± 0.13* | 28.26 ± 1.81* | 0.97 ± 0.08 | 5.61 ± 0.13ab |
ALK-RNAi#3 | 1.49 ± 0.20* | 16.08 ± 1.96* | 1.59 ± 0.15 | 2.66 ± 0.19* | 6.74 ± 0.55 | 0.63 ± 0.39 | 1.08 ± 0.34 | 30.53 ± 3.66* | 0.90 ± 0.16 | 5.72 ± 0.42ab |
ALK-RNAi#16 | 2.01 ± 0.16* | 18.22 ± 1.78* | 2.04 ± 0.45 | 3.09 ± 0.19* | 6.23 ± 0.35 | 0.13 ± 0.38 | 1.19 ± 0.06 | 32.97 ± 2.46* | 0.70 ± 0.01 | 5.33 ± 0.34ab |
ALK-RNAi#27 | 2.31 ± 0.07* | 22.11 ± 0.31* | 1.68 ± 0.12 | 4.45 ± 0.15* | 6.85 ± 1.52 | 0.21 ± 0.26 | 0.78 ± 0.40 | 38.54 ± 1.26* | 0.73 ± 0.05 | 5.43 ± 0.31ab |
Concentration of individual glucosinolates and total phenol content in the leaves of ALK-RNAi lines (T1) used for fungal infectivity assay is given. Asterisk indicates significant increase in glucosinolate compared to the wild-type control. Lettera on top indicate significant difference in mycelial diameter compared to mock (methanol) and lettersab represent significant difference from both mock and wild-type control. Statistical analysis was conducted using one-way ANOVA following Fishers LSD test of significance at P < 0.05.