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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Endocr Relat Cancer. 2016 Jul 8;23(9):677–690. doi: 10.1530/ERC-16-0136

Figure 7. In vitro effects of Kynurenine and 2-hydroxyglutarate in human breast cancer MCF7 cells.

Figure 7

A. Treatment protocols for MCF7 cells with kynurenine (Kyn) or 2-hydroxyglutarate (2-OHG) in Dox-treated cells. Dox-treated cells without (−) and with (+) added Kyn (10 μM) were evaluated for apoptotic status (B), cell viability (C), and mammosphere-formation ability (D). Results (mean ± SEM) are from 3 independent experiments. For C and D, each experiment was conducted in quadruplicates. *P < 0.05 between treatment groups. Dox-treated cells were similarly treated with 2-OHG (10 μM) and analyzed for apoptotic status (E), cell viability (F), and mammosphere-formation ability (G), following described protocols (A). Results (mean ± SEM) are from 3 independent experiments. For F and G, each experiment was conducted in quadruplicates. *P < 0.05 between treatment groups.