Figure 2. Protective effect of SAL against the CoCl₂-induced loss of MG-63 and ROB cell differentiation.

MG-63 or ROB cells were cultured in 1% FBS in the presence of either SAL (1, 10, 100 or 1000 nM) or vehicle, followed by treatment with CoCl₂ for 2, 4, or 6 days. After SAL treatment, ALP activity was measured in MG-63 cells (a) or ROB cells (d) using an ALP activity assay kit. After SAL treatment, the level of type I collagen secretion in the cell culture supernatants of MG-63 cells (b) or ROB cells (e) was analysed by ELISA. After SAL treatment, the production of osteocalcin in the cell culture supernatants of MG-63 cells (c) or ROB cells (f) was detected by ELISA. *P < 0.05, **P < 0.01 compared with vehicle control; ^##P < 0.01 compared with CoCl₂.