Table 1. Strains and plasmids used in this study.
| Strains and plasmids | Description | Source |
|---|---|---|
| HD-1 | L-phenylalanine overproducing strain. It was derived from E. coli W3110 strain, which initially undergone multiple random mutagenesis and then overexpressing aroFWT and pheAfbr. The genome sequences of the HD-1 strain were determined and compared (Supplementary Tables 2–3). The genome of this strain was used as the template for amplification of phenylalanine synthesis genes | This study |
| DH5a | This strain was used for the clone of the plasmid. | Lab stock |
| BL21 (DE3) | This strain was used for enzyme protein expression. | Lab stock |
| HD-C1 | HD-1 p15A1-aroC1::aroC | This study |
| HD-C2 | HD-1 p15A1-aroC2::aroC | This study |
| HD-C3 | HD-1 p15A1-aroC3::aroC | This study |
| HD-L1 | HD-1 p15A1-aroL1::aroL | This study |
| HD-L2 | HD-1 p15A1-aroL2::aroL | This study |
| HD-L3 | HD-1 p15A1-aroL3::aroL | This study |
| HD-A1 | HD-1 p15A1-aroA1::aroA | This study |
| HD-A2 | HD-1 p15A1-aroA2::aroA | This study |
| p15A1 | p15A derivative, carrying pheAfbr aroFwt gene, Kanr | Lab stock |
| pET28-a(+) | E. coli expression plasmid, T7 promoter, T7 terminator, Kanr | Lab stock |
| pET28a-aroL | N-terminal His-tagged aroL, inserted between BamHI and EcoRI sites of pET 28a (+) | This study |
| pET28a-aroK | N-terminal His-tagged aroK, inserted between BamHI and EcoRI sites of pET 28a (+) | This study |
| pET28a-aroA | N-terminal His-tagged aroA, inserted between BamHI and EcoRI sites of pET 28a (+) | This study |
| pET28a-aroC | N-terminal His-tagged aroC, inserted between EcoRI and HindIII sites of pET 28a (+) | This study |
| pET28a-pheA | N-terminal His-tagged pheA, inserted between BamHI and EcoRI sites of pET 28a (+) | This study |
| pET28a -tyrB | N-terminal His-tagged tyrB, inserted between BamHI and, EcoRI sites of pET 28a (+) | This study |
| p15A1-aroC1 | p15A-aroFwt-pheAfbr derivative, carrying aroC gene, BBa_J23116 promoter, Kanr | This study |
| p15A1-aroC2 | p15A-aroFwt-pheAfbr derivative, carrying aroC gene, BBa_J23107 promoter, Kanr | This study |
| p15A1-aroC3 | p15A-aroFwt-pheAfbr derivative, carrying aroC gene, BBa_J23118 promoter, Kanr | This study |
| p15A1-aroL1 | p15A-aroFwt-pheAfbr derivative, carrying aroL gene, BBa_J23116 promoter, Kanr | This study |
| p15A1-aroL2 | p15A-aroFwt-pheAfbr derivative, carrying aroL gene, BBa_J23107 promoter, Kanr | This study |
| p15A1-aroL3 | p15A-aroFwt-pheAfbr derivative, carrying aroL gene, BBa_J23118 promoter, Kanr | This study |
| p15A1-aroA1 | p15A-aroFwt-pheAfbr derivative, carrying aroA gene, BBa_J23116 promoter, Kanr | This study |
| p15A1-aroA2 | p15A-aroFwt-pheAfbr derivative, carrying aroA gene, BBa_J23107 promoter, Kanr | This study |
| p15A1-aroA3 | p15A-aroFwt-pheAfbr derivative, carrying aroA gene, BBa_J23118 promoter, Kanr | This study |
Kan, kanamycin; r, resistance.