Table 2. The determination of purified enzyme activity.
| Enzyme fraction | Phenylpyruvate formed (μM) | |
|---|---|---|
| Control | aroL, aroA | 0 |
| Part 1 | aroL, aroA, aroC, pheA | 0.26 |
| Part 2 | tyrB | 0.32 |
Incubation mixture was described in the “Assay of the enzyme activities”. Phenylpyruvate was determined as described in the “Determination of phenylpuruvate acid”.