Selection of the Optimal Herbal Compositions of Red Clover and Pomegranate According to Their Protective Effect against Climacteric Symptoms in Ovariectomized Mice

. 2016 Jul 23;8(8):447. doi: 10.3390/nu8080447

Points Groups	Food Consumption (g/24 h/Mouse): Days after Initial Treatment
Points Groups	1	3	7	28	56	83
Controls
Sham	7.20 ± 0.72	6.35 ± 0.67	6.29 ± 0.72	6.59 ± 0.77	7.31 ± 0.90	7.54 ± 0.78
OVX	11.40 ± 1.44 *	11.11 ± 0.98 *	11.29 ± 1.03 *	11.38 ± 1.33 *	12.80 ± 1.41 *	14.75 ± 1.57 *
Estradiol	11.49 ± 1.41 *	10.53 ± 0.93 *	9.82 ± 0.93 *^,#	9.52 ± 1.01 *^,#	10.24 ± 0.98 *^,#	10.65 ± 1.54 *^,#
RC	11.26 ± 1.18 *	11.01 ± 1.53 *	11.06 ± 1.06 *	11.11 ± 0.92 *	12.66 ± 1.62 *	14.60 ± 1.08 *
PCP	11.91 ± 1.38 *	11.36 ± 1.67 *	11.52 ± 1.20 *	11.26 ± 1.31 *	12.87 ± 1.55 *	14.67 ± 1.34 *
RC:PCP
1:1	11.46 ± 1.12 *	10.98 ± 1.34 *	11.13 ± 1.24 *	11.29 ± 1.31 *	12.43 ± 1.36 *	14.52 ± 1.40 *
1:2	11.08 ± 1.19 *	10.96 ± 1.25 *	11.33 ± 1.25 *	11.57 ± 1.19 *	13.04 ± 0.91 *	14.79 ± 1.35 *
1:4	11.24 ± 1.13 *	11.34 ± 1.11 *	11.58 ± 1.32 *	11.84 ± 1.44 *	12.45 ± 0.84 *	14.38 ± 1.68 *
1:8	11.62 ± 1.01 *	11.17 ± 1.40 *	11.37 ± 1.55 *	11.43 ± 1.35 *	12.95 ± 0.92 *	14.65 ± 2.04 *
2:1	11.66 ± 0.93 *	11.25 ± 0.95 *	11.04 ± 1.61 *	11.31 ± 1.14 *	12.90 ± 1.33 *	14.59 ± 1.62 *
4:1	11.42 ± 1.46 *	11.25 ± 0.99 *	11.40 ± 1.26 *	11.34 ± 1.26 *	12.76 ± 1.17 *	14.83 ± 1.23 *
8:1	11.34 ± 1.99 *	11.13 ± 1.13 *	11.37 ± 1.58 *	11.46 ± 1.25 *	12.86 ± 0.72 *	14.89 ± 1.17 *

Values are expressed mean ± S.D. of eight mice. * p < 0.05 as compared with sham control, ^# p < 0.05 as compared with OVX control. OVX = Bilateral ovariectomy, RC = Red clover dry extracts, PCP = Pomegranate Concentrate Powder, RC:PCP = Mixed formulations consisted of RC and PCP (g/g).