Table 3.
Primer sequences of the target and reference genes.
| Gene | Primers | Sequence | Accession Number | Product Size (bp) |
|---|---|---|---|---|
| gpX | Forward | 5′-GCTCGGTGTATGCCTTCTCT-3′ | NM_214201.1 | 103 |
| Reverse | 5′-AGCGACGCTACGTTCTCAAT-3′ | |||
| Gr | Forward | 5′-CTACGTGAGCCGACTGAACA-3′ | AY368271.1 | 146 |
| Reverse | 5′-TCAGGATGTGAGGAGCTGTG-3′ | |||
| CuZn-sod | Forward | 5′-GAGACCTGGGCAATGTGACT-3′ | GU944822.1 | 139 |
| Reverse | 5′-CTGCCCAAGTCATCTGGTTT-3′ | |||
| Mn-sod | Forward | 5′-TGGAGGCCACATCAATCATA-3′ | NM_214127.2 | 136 |
| Reverse | 5′-AGCGGTCAACTTCTCCTTGA-3′ | |||
| e-sod | Forward | 5′-ACGCTGCTCTGTGCTTACCT-3′ | NM_001078688.1 | 142 |
| Reverse | 5′-TCAACTCCTGCCAGATCTCC-3′ | |||
| Aat | Forward | 5’-CTCGGGTGTCACAGAGGAAC-3’ | X88780.1 | 132 |
| Reverse | 5’-GTGGCCTCTGTCCCTTTCTC-3’ | |||
| Gen | Forward | 5’-CACTACTGGCTGGGCAATGA-3’ | X13871.1 | 87 |
| Reverse | 5’-ATCCTGATGCCACTCCTCCT-3’ | |||
| Afp | Forward | 5’-GCACGAAGAGATGCCCATAAAC-3’ | NM214317.1 | 116 |
| Reverse | 5’-ATGTCTCATCCAACACCAAGCT-3’ | |||
| grp78 | Forward | 5’-AACCAAGGACGCTGGAACTATT-3’ | X92446.1 | 181 |
| Reverse | 5’-AACACCAGGATGTTCTTCTCCC-3’ | |||
| lhx8 | Forward | 5’-GCTGTCTCCACCCATGTTAGAA-3’ | FJ587986.1 | 92 |
| Reverse | 5’-CATCCATGTAACTATGCAGCGC-3’ | |||
| Nobox | Forward | 5’-CCAAGAAGACCACTATCCGGAC-3’ | FJ587509.1 | 150 |
| Reverse | 5’-GTGTCCTTGTTCTCCTTCCCAT-3’ | |||
| sohlh2 | Forward | 5’-TTGAACGTGTACTCTGTCCCTG-3’ | XM_013980443.1 | 147 |
| Reverse | 5’-AGCATGGAAGGAATTGAGAGCA-3’ | |||
| bcl2 | Forward | 5’-CTTGGAGGGGACACTCTTCTTC-3’ | XM_003121700 | 140 |
| Reverse | 5’-TTTCTCATCACTGTCCTTCGGG-3’ | |||
| Bax | Forward | 5’-TTCAGGGTTTCATCCAGGATCG-3’ | XM_003127290 | 157 |
| Reverse | 5’-ATCCTCTGCAGCTCCATGTTAC-3’ | |||
| cas3 | Forward | 5’-TGGCGTGTCAGAAAATACCAGT-3’ | NM_214131.1 | 94 |
| Reverse | 5’-GATCCGTCCTTTGAATTTCGCC-3’ | |||
| β-actin | Forward | 5’-GGCCGCACCACTGGCATTGTCAT-3’ | DQ845171.1 | 104 |
| Reverse | 5’-AGGTCCAGACGCAGGATGGCG-3’ |
gpx, glutathione peroxidase; gr, glutathione reductase; CuZn-sod, cytoplasmic copper/zinc superoxide dismutase; Mn-sod, manganese superoxide dismutase; g-sod, extracellular superoxide dismutase; aat, alpha-1-antitrypsin; gen, gelsolin; afp, alpha-fetoprotein; grp78, 78-kDa glucose-regulated protein; lhx8, LIM homeobox protein 8; nobox, newborn ovary homeobox gene; sohlh2, spermatogenesis and oogenesis-specific basic helix-loop-helix 2; bcl2, B-cell lymphoma-2; bax, Bcl-2 assaciated X protein; cas3, caspase-3.