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. 2016 Aug 4;115(5):553–563. doi: 10.1038/bjc.2016.172

Figure 4.

Figure 4

Synergistic induction of CXCL9 and CXCL10 by IFN-γ and TNF-α in human ovarian cancer cell lines. OV-MZ-6 (A) and SKOV-3 (B) cells were stimulated with increasing concentrations of IFN-γ and TNF-α as indicated. In both cell lines, both CXCL9 (left panels) and CXCL10 (right panels) were induced by IFN-γ stimulation in a dose-dependent manner, whereas TNF-α had this effect only on CXCL10 secretion. (C–E) Cytokine-induced CXCL9 and CXCL10 secretion from ovarian cancer cells is dependent on the JAK/STAT and NF-κB signalling. Starved OV-MZ-6 cells were stimulated with IFN-γ or TNF-α with increasing concentrations of JAK inhibitor I (C) or the NF-κB inhibitors TPCK (D) or BAY 11–7082 (E) for 24 h. SKOV-3 cells revealed analogous results (data not shown). Asterisks mark significant differences compared with controls unless otherwise indicated.