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. 2016 Jul 28;8(8):209. doi: 10.3390/v8080209

Figure 3.

Figure 3

NXT1 knockdown accumulates viral NP and M1 proteins in the nucleus. (A–C) MDCK cells were transfected with control or NXT1 siRNA for 48 h and infected with influenza A/WSN/33 virus at MOI = 2. After 2, 4, or 6 hpi, the cells were analyzed by immunofluorescent staining with anti-NP (A); anti-M1 (B); or anti-NEP (C) antibody, followed by Alexa Fluor 594 goat anti-mouse or Alexa Fluor 594 goat anti-rabbit antibody. Nuclei were counterstained with Hoechst 33342 and the cells were visualized under an Olympus laser scanning confocal fluorescence microscope. White arrows indicate nuclear accumulation of NP or M1 proteins; (D–F) Mean fluorescence intensity of NP, M1, and NEP in the nucleus derived from (A–C) (normalized by the mean fluorescence intensity of Hoechst 33342, n ≥ 50). Data represent the mean ± SD values from three independent experiments. * indicates a significant difference at p < 0.05 (unpaired t-test).