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Design strategies for replication-competent fluorescent or bioluminescent reporter viruses. Additional transcription units (ATUs) can be inserted 3’ of the first gene in the viral genome (a) or 5’ of the final gene in the viral genome; between viral genes (b); or their sequences can be fused with viral genes to generate fusion proteins (FPs) either at the N-terminus or C-terminus (c) of a viral gene; or at inert hinge regions within a viral protein (d).
