Table 1.
CDK/cyclin-specific proteins (selected) including viral protein kinase pUL97 coimmunoprecipitated with antibodies against endogenous cyclins B1, T1 and H using lysates of transiently pUL97-transfected 293T cells *.
| Gene | Entry | Length | Protein | WSC [bait]/WSC [Fc-control] | |||
|---|---|---|---|---|---|---|---|
| B1 | T1 | H | pUL97 | ||||
| CDK5 | Q00535 | 292 | Cyclin-dependent like kinase 5 | 5 | - | - | - |
| CCNB1 | P14635 | 433 | Cyclin B1 | 34 | - | - | 5 |
| CDK1 | P06493 | 297 | Cyclin-dependent kinase 1 | 49 | - | - | 8 |
| CDK2 | P24941 | 298 | Cyclin-dependent kinase 2 | 5 | - | - | b.c. |
| CCNT1 | O60563 | 726 | Cyclin T1 | - | 49 | - | - |
| CDK9 | P50750 | 372 | Cyclin-dependent kinase 9 | 4 | 41 | - | 4 |
| CCNT2 | O60583 | 730 | Cyclin T2 | - | 5 | - | - |
| CCNH | P51946 | 323 | Cyclin H | - | - | 47 | - |
| CDK7 | P50613 | 346 | Cyclin-dependent kinase 7 | - | - | 27 | - |
| MNAT1 | P51948 | 309 | Cyclin H assembly factor | - | - | 17 | - |
| AFF4 | Q9UHB7 | 1163 | Major CDK9 EF-associated protein | 3 | 20 | b.c. | 12 |
| UL97 | P16788 | 707 | HCMV protein kinase pUL97 | 27 | 4 | 5 | 34 |
* The 293T cells transiently expressing pUL97-Flag were harvested two days post-transfection for mass spectrometry analyses. Rabbit Fc fragment was used as the reference control for CoIP specificity; b.c., below cut-off (cut-off ≥ 3 and 3-fold enrichment compared to control sample); WSC, weighted spectral counting.