Figure 5. Effects of YD on uPA expression in H292-Gef cells.

(a) H292-Gef cells were treated with 10 nM YD for the indicated times. The protein levels of SerpinB2 and uPA were examined by western blot analysis. (b) The mRNA levels of SERPINB2 and UPA were determined in H292-Gef cells following 24 h treatment with 10 nM YD. β-actin was used for normalization. The data are presented as the mean fold changes ± SD relative to the SERPINB2 of control. (c) Following treatment with 10 nM YD for 6 h, H292-Gef cells were fixed, blocked and stained with anti-SerpinB2, anti-uPA, anti-LAMP1 and DAPI. Images were taken using a confocal laser microscope. Scale bar, 10 μm. Boxed regions in merged images are enlarged and shown below each markers. (d,e) The co-localization of indicated markers were quantitatively analysed using the LAS AF Image software (Leica), and expressed as the percentages of co-localization rate (d) and Pearson’s coefficients (e). The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.005. Comparison between Pearson’s coefficients in control and 10nM YD treated cells were statistically analysed by unpaired Student’s t-test, ^#P < 0.0001.