Figure 2. Wedelolactone regulats osteoblast differentiation through Wnt/GSK3β/β-catenin pathway.

(A) Wedelolactone enhanced the phosphorylation of GSK3β, and nuclear accumulation of β-catenin and runx2. BMSC were incubated with or without wedelolatone for 9 d, and then cytosolic lysates and nuclear lysates were subjected to immunoblotting with antibodies as indicated. (n = 3) (B) The inhibitory effect of wedelolatone on GSK3β activity. Left: Different concentrations of wedelolactone or staurosporin were incubated with the reaction solution, and then GSK3β activity was assayed. Right: Cartoon representation of wedelolactone docked onto the crystal structure of GSK3β. (n = 3). Error bars (A,B) denote mean ± SD. *P < 0.05; **P < 0.01.