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. 2016 Aug 25;16(1):681. doi: 10.1186/s12885-016-2724-0

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© Li et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — IHC staining of Tiam1 protein in breast cancer tissues. a Lack of Tiam1 protein staining in normal breast tissues. b Tiam1 protein was showed weakly positive in DCIS tissues. c Diffuse and strong positive expression of Tiam1 protein staining in breast cancer cells with LN metastasis (Luminal B). d Tiam1 was weakly positive in breast cancers with LN metastasis (Luminal B). e Lack of Tiam1 protein staining in breast cancers without metastasis (triple negative). f Tiam1 protein staining localized some nuclear staining was also observed in the metastatic breast cancers with lymphatic vessel invasion (triple negative). (Original magnification, A-F: ×200)