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Tooth morphogenesis is disrupted in Pitx2HD mutants. (A–C) Ventral views of alizarin red staining of teeth at 5 dpf in wild-type (A), pitx2HDsny7/sny7 (B) and pitx2csny3/sny3 (C) fish. c5: fifth ceratobranchial; 4V1, 3V1 and 5V1 designate individual teeth. (D) Quantification of tooth defects observed in pitx2HDsny7 and pitx2csny fish at 5 dpf. Tooth malformations were defined as a reduced number of teeth and incomplete tooth formation as shown in (B).
