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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Dev Biol. 2016 Jun 11;416(1):69–81. doi: 10.1016/j.ydbio.2016.06.010

Fig. 7.

Fig. 7

Asymmetric expression of elovl6 depends on Kupffer’s vesicle. (A) RNA in situ hybridizations revealed similar asymmetric expression patterns for pitx2c and evolv6 in left lateral plate mesoderm (black arrows) and brain (arrowheads) at 18–20 somite stages (ss). pitx2c mRNA was detected in hatching gland (asterisks) and neurons (white arrows), whereas elovl6 was not expressed in these domains. White dashed line is the midline; L=left and R=right. (B) Representative images are shown of left-sided, bilateral, right-sided and absent elovl6 expression in lateral plate mesoderm (arrows) at 18 somite stage. The graph shows quantification of asymmetric elovl6 expression profiles in control, Lgl2 depleted and genotyped cftrpd1048 mutant embryos.