TABLE 3.
Summary of the genes related to conjugative transfer
| Plasmid name (GenBank accession no.) | Length (bp) | Mobility gene(s) |
T4SS MPF gene(s)b | Plasmid transferability | Plasmid/contig profile of:d |
Similarity (% [WSS])c | |||
|---|---|---|---|---|---|---|---|---|---|
| Relaxase gene(s) | T4CPa | A. xylosoxidansKUN4507 | K. pneumoniae KUN4843 | K. pneumoniae KUN5033 | |||||
| pKUN4507_1 (LC155906) | 26,345 | nikB, mobC | trwB | virB5, virB6, traG | Mobilizable | + | − | + | 100 (100)e |
| pKUN4507_2 (LC155907) | 19,889 | traD | traA, traC, traG | Nontransferable | + | − | − | ||
| pKUN4843_1 (LC155908) | 38,744 | Nontransferable | − | + | + | 100 (99.99)f | |||
| pKUN4843_2 (LC155909) | 34,541 | mobC | virD4 | virB2, virB4, virB5, virB6, virB8, virB9, virB10, virB11, virD2, traC | Conjugative | − | + | + | 100 (100)f |
| pKUN4843_others | 103,616 | − | + | + | |||||
| Contig 22 | 57,649 | − | + | +h | 100f,g | ||||
| Contig 29 | 22,103 | − | + | +h | 100f,g | ||||
| Contig 34 | 8,638 | − | + | +h | 100f,g | ||||
| Contig 35 | 8,329 | finO, traA, traE, traI, traJ, traK traL, traM, traX, traY | − | + | +h | 100f,g | |||
| Contig 39 | 5,844 | − | + | +h | 100f,g | ||||
| Contig 53 | 1,053 | − | + | +h | 99.0f,g | ||||
a
Type IV secretion system coupling protein.
b
T4SS, type IV secretion system; MPF, mating pair formation.
c
Sequence similarity was determined using mapping analysis, and whole-sequence similarity (WSS) of circularized plasmids was calculated using ClustalW.
d
Presence (+) or absence (−) of the plasmid/contig is indicated.
e
Sequence similarity between A. xylosoxidans KUN4507 and K. pneumoniaeKUN5033.
f
Sequence similarity between K. pneumoniae KUN4843 and K. pneumoniae KUN5033.
g
Excluding the regions where depth of coverage is <10%.
h
The Illumina reads of K. pneumoniae KUN5053 could be mapped to this contig of KUN4843.