Skip to main content
. 2016 Aug 22;60(9):5189–5197. doi: 10.1128/AAC.00539-16

FIG 2.

FIG 2

Supercoiling activities of recombinant M. tuberculosis GyrA and GyrB at 1 unit in the presence of relaxed pBR322 DNA and ATP. Lane 1, relaxed pBR322 DNA alone (negative control); lane 2, E. coli GyrA2B2 complex (Inspiralis) (positive control); lane 3, recombinant WT GyrA alone; lane 4, recombinant WT GyrB alone; lane 5, recombinant WT GyrA and GyrB complex; lane 6, recombinant GyrB-N538D alone; lane 7, recombinant WT GyrA and GyrB-N538D complex; lane 8, recombinant GyrB-E540D alone; lane 9, recombinant WT GyrA and GyrB-E540D complex. R and Sc, relaxed and supercoiled pBR322 DNA, respectively.