TABLE 1.
Nucleotide sequences of the primers used in this study
| Primer | Nucleotide sequencea | Comment |
|---|---|---|
| gyrB_Fw | 5′-GCACCAGGAAGAAAGATGTCC-3′ | Amplification (nucleotide positions 5084–5104)b |
| gyrA_Rv | 5′-TTCCTCCTCAGATCGCTACG-3′ | Amplification (nucleotide positions 9867–9848)b |
| gyrA_cln_Fw | 5′-ATGACAGACACGACGTTGCCGCCTG-3′ | Cloning, bp 1–25 of gyrA |
| gyrA_cln_Rv | 5′-CATCGTCGTCGCTCGAGCCTGATTAA-3′ | Cloning, inserted XhoI site |
| gyrB_cln_Fw | 5′-ATGGGTAAAAACGAGGCCAGAAGATC-3′ | Cloning, bp 1–25 of gyrB |
| gyrB_cln_Rv | 5′-TGCATCTCCTGCAGGATGTCAACCG-3′ | Cloning, inserted SbfI site |
| Mut_GB538_Fw | 5′-GGTGCTAAAGGACACCGAAGTTCAG-3′ | Mutagenesis |
| Mut_GB538_Rv | 5′-CGGTCGATGCGCGCTTTC-3′ | Mutagenesis |
| Mut_GB540_Fw | 5′-AGAACACCGACGTTCAGGCGATC-3′ | Mutagenesis |
| Mut_GB540_rv | 5′-TTAGCACCCGGTCGATGC-3′ | Mutagenesis |
a
The restriction enzyme site or mutated nucleotide is underlined.
b
Nucleotide position on chromosome of M. tuberculosis H37Rv (accession no. NC_000962.3).