FIG 3.

Inhibitory effect of mitoxantrone on lamivudine transport by MATE1. (A) IC₅₀s reflecting inhibition of ASP⁺ uptake into OCT1-, OCT2-, and MATE1-expressing MDCK cells by mitoxantrone. The IC₅₀s with 95% confidential intervals were calculated from three independent measurements. (B and C) Effect of 2 μM mitoxantrone on transcellular transport (B) and intracellular accumulation (C) of lamivudine (100 nM) in monolayers of MATE1-expressing and control cells. Data were analyzed by two-way ANOVA with multiple comparisons (**, P < 0.01, ***, P < 0.001, versus respective noninhibited controls) and are shown as means ± SD (n ≥ 3). n.s., not significant.