Dosing and toxicity of Q3G. Naive C57BL/6 male mice were dosed with 12.5 to 400 mg/ml Q3G or 10% DMSO intraperitoneally. (A and B) Survival (A) and changes in body weight (B) were monitored for 14 days as an indication of toxicity. (C) Experimental plan outlining treatment. Six- to eight-week-old female BALB/c mice were treated with 50 mg/kg Q3G (n = 10 per group) or 10% DMSO (n = 10) via intraperitoneal injection. Mice in all groups received a challenge dose of 1,000× LD50 of mouse-adapted Ebola virus (Mayinga isolate) in 200 μl PBS (pH 7.4) by intraperitoneal injection. (D and E) Survival (D) and changes in weight (E) of Q3G-treated and untreated mice. (F) Experimental plan outlining treatment. Six- to eight-week-old female BALB/c mice received a challenge dose of 1,000× LD50 of mouse-adapted Ebola virus (Mayinga isolate) in 200 μl of PBS (pH 7.4) by intraperitoneal injection. At 24 h postinfection, mice were treated with 50, 100, or 200 mg/kg of Q3G (n = 10) or 10% DMSO (n = 10) every other day via intraperitoneal injection. (G and H) Survival (G) and changes in weight (H) of Q3G-treated and untreated mice.