FIG 7.

Cos-Seq analysis of L. infantum carrying an L. infantum gDNA cosmid library selected under standard growth conditions (Ø selection) (A) and with antimonyl tartrate (Sb^III) (B), sodium arsenite (As^III) (C), copper acetate (Cu²⁺) (D), cadmium acetate (Cd²⁺) (E), and miltefosine (F) at their IC₅₀s. The same transfected population was also used to infect bone marrow-derived macrophages, which were selected by in vitro infection only (G), under miltefosine (H), or under sodium stibogluconate (Sb^V) (I). In vitro macrophage passages under conditions of drug selection were repeated three times. Cosmid DNA was recovered from all selected populations and subjected to next-generation sequencing. Sequence reads were then aligned with the sequence of L. infantum chromosome 34. In panels A to I, the alignment frequency (y axis) is plotted against chromosomal positions (x axis). (J) Scatter graph analysis of alignment frequencies at positions 36000 to 75000 on chromosome 34 for next-generation sequencing reads from selected intracellular amastigotes (G to I). The black bars represent the median alignment frequencies. *, P < 0.05; **, P < 0.01.