TABLE 1.

Quantification of cell death markers^a

Sample no.	Construct	Miltefosine concn (μM)	SbIII concn (μM)	% cellsb:
				Annexin V−, PI−	Annexin V+, PI−	Annexin V+, PI+	Annexin V−, PI+
1	pCLN	0		99.0	0	1	0
2	ARM58	0		99	0	0	1
3	pCLN	80		50.4	27.1	17.8	4.76
4	ARM58	80		36.80	33.30	26.70	3.16
5	pCLN		0	99	0	0.60	0
6	ARM58		0	99	0	0.60	0
7	pCLN		400	89.5	4.6	2.4	3.4
8	ARM58		400	97.3	0.6	1.2	1.0
9	pCLN		800	75.9	5.7	8.2	10.2
10	ARM58		800	90.6	6.3	1.6	1.5

^aL. donovani promastigotes with pCLN or ARM58 transgenes were challenged with 80 μM miltefosine or 400 to 800 μM Sb^III for 72 h. Cells were stained with the cell death markers annexin V and propidium iodide (PI), and 10,000 events were analyzed by fluorescence-assisted cell sorting. Raw data can be found in Fig. S1 in the supplemental material.

^bAnnexin V-negative (annexin V⁻) and PI-negative (PI⁻) cells are viable, annexin V-positive (annexin V⁺) and PI-negative cells are in early PCD, annexin V-positive and PI-positive (PI⁺) cells are in PCD, and annexin V-negative and PI-positive cells are ruptured.