Fig. (6).

Social interaction test. PV-Cre mice were injected with the lentiviral control vector or lentiviral DIO-hM3D-Gq-mCherry vector. Thirty minutes before behavioral testing, mice were given CNO (0.5 mg/kg body weight) by intraperitoneal injection. (A) In the first session of the social interaction test, the average duration of time (sec) spent in the chamber with the Stranger 
1 mouse or with the empty cup (empty) was recorded. Both of the cherry and hM3D-Gq group mice preferred the stranger 
1 mouse. Statistical analysis was conducted using two-way analysis of variance (ANOVA). Treatment vs chamber F(1,84)=0.350, P=0.556; treatment F(1,84)=5.922, P=0.017; chamber F(1,84)=11.768, P=0.001. (B) In the second session, the average duration of time (sec) spent in the chamber with the Stranger 1 mouse or with a new unfamiliar mouse (Stranger 2) was recorded. n = 22 for the control group; n = 22 for the hM3D-Gq group. Control group, P=0.033; hM3D-Gq group, P=0.947. Treatment×chamber F(1,84)=2.016, P=0.159; F(1,84)=8.806, P=0.004; chamber F(1,84)=0.982, P=0.325.