FIGURE 1.

Schematic organization of the pneumococcal pezAT operon. The scheme depicts the position of the single promoter sequence (line with arrowhead pointing to the direction of transcription), which includes the -35 and -10 regions, as well as the inverted repeated sequence (PS, convergent arrows) that is the binding site of PezA and PezT proteins. Ribosome-binding site sequences are denoted as RBS and the initial start site is also depicted with arrow. The pezA termination codon, and the overlapping pezT initiation codon are also indicated. Substitution of the entire operon by a gene cassette encoding resistance to kanamycin is shown below.