TGFβ1 and TGFβ3 Are Required for Specification of HSCs
(A) Expression of runx1 in wild-type, tgfb1, tgfb2, and tgfb3 morphants.
(B) Expression of kdrl in wild-type, tgfb1, tgfb2, and tgfb3 morphants.
(C) Expression of dll4 in wild-type, tgfb1, tgfb2, and tgfb3 morphants.
(D) Expression of dlC in wild-type, tgfb1, tgfb2, and tgfb3 morphants. All samples were analyzed at 28 hpf.
(E–G) Maximum projections of itga2b:GFP; Kdrl:HRas-mCherry transgenic embryos in (E) uninjected, (F) tgfb1 morphants, and (G) tgfb3 morphants at 48 hpf. The images show part of the trunk DA and white arrowheads denote itga2b:GFP+ (green), kdrl:HRas-mCherry+ (magenta) HSPCs.
(H) HSPCs counts in the entire trunk region of uninjected, tgfb1, and tgfb3 morphant itga2b:GFP; Kdrl:HRas-mCherry transgenic embryos at 48 hpf (p value is indicated on the graph, n = 10 for each of the conditions).
(I–K) Expression of rag1 in the thymus at 4 dpf (red arrowheads) in (I) wild-type, (J) tgfb1 morphants, and (K) tgfb3 morphants.
(L) Expression of efnB2a in wild-type, tgfb1, and tgfb3 morphants.
(M) Expression of gata2b in wild-type, tgfb1, and tgfb3 morphants. The numbers of embryos are shown in each panel as the number of embryos with phenotype/total number analyzed.
See also Figure S3.