Figure 6.

Cytotoxicity analysis.

Notes: The RCV of TVECs labeled with CL-PEG-MnFe₂O₄ within 0.9 mM decreased only 7%–9% compared to controls. As the concentration increased, the RCV decreased correspondingly, and a significant decrease of ~19% in viability of TVECs incubated with 4.5 mM CL-PEG-MnFe₂O₄ solution was measured. On the contrary, a notable decrease of ~15% in viability of TVECs incubated with only 0.05 mM MnCl₂ solution was observed. When the TVECs were incubated with MnCl₂ solution as high as 4.5 mM, there were only 36.2% cells that survived.

Abbreviations: NMs, nanomicelles; PEG, poly(ethylene glycol); RCV, relative cell viability; TVECs, tumor vascular endothelial cells.