Figure 7.

Confocal laser scanning microscopy of cell uptake of HaCaT cell after 4 hours of administration of (A) DMEM; (B) C6 aqueous suspension; (C) C6–NIC complex suspension; (D) C6–HA–Chol–NP suspension; and (E) C6–HA–Chol–NPs–NIC suspension.

Notes: The concentration of C6 in each formulation was 10 μg/mL. Cells were stained for nuclei (in blue) and C6 (in green). Scale bar: 20 μm. Magnification ×400.

Abbreviations: C6, coumarin 6; C6–HA–Chol–NP, coumarin 6-loaded hyaluronic acid–cholesterol nanoparticle; C6–HA–Chol–NPs–NIC, coumarin 6-loaded hyaluronic acid–cholesterol nanoparticles containing nicotinamide; C6–NIC complex, coumarin 6–nicotinamide complex; DAPI, 4′,6-diamidino-2-phenylindole; DMEM, Dulbecco’s Modified Eagle’s Medium; h, hours; HaCaT, human keratinocytes.