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. 2016 Jul 13;12(3):1861–1867. doi: 10.3892/ol.2016.4850

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Copyright: © Yi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of CRT overexpression and CRT downregulation on proliferation of HL-60 cells induced by DADS. Inhibition of CRT expression by siRNA and CRT overexpression by plasmid transfection in HL-60 cells were evaluated by (A) reverse transcription-polymerase chain reaction: 1, HL-60; 2, HL-60/CRT; 3, HL-60/CRT siRNA; 4, DADS+HL-60; 5, DADS+HL-60/CRT siRNA; 6, DADS+HL-60/CRT (*P<0.05 vs. control cells) and (B) western blot analysis: 1, HL-60; 2, HL-60/CRT; 3, HL-60/CRT siRNA; 4, DADS+HL-60; 5, DADS+HL-60/CRT siRNA; 6, DADS+HL-60/CRT. (C) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide analysis in CRT-overexpressing and CRT-downregulated HL-60 cells, with and without DADS treatment. *P<0.05 vs. control cells. CRT, calreticulin; DADS, diallyl disulfide; siRNA, small interfering RNA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.