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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Microbiol. 2016 Jun 10;101(4):625–644. doi: 10.1111/mmi.13414

Figure 4.

Figure 4

Binding of RedRock ParB to parSA. Binding of RedRock ParB to parS-L and parS-R. ParB was incubated at different concentrations to a 45 bp nonspecific DNA fragment (left), a 666 bp fragment containing parS-L (middle) and a 194 bp fragment containing parS-R (right), and the complexes separated from unbound DNA by native acrylamide gel electrophoresis. Protein concentrations from left to right are : 0, 8.4, 25, 76, 228, 684, 2054, 6163, and 18490 nM. B. ParB binding to DNA fragments (from left to right) containing all 8 repeats of parS-L (60 bp), the four leftmost repeats (38 bp), the four rightmost repeats (38 bp), and the four leftmost repeats with flanking nonspecific DNA (60 bp). Protein concentrations are as in panel A. C. ParB binding to DNA fragments containing varying numbers of 8 bp repeats. From left to right, increasing the parS-L repeat from 1 to 4. Protein concentrations are as in panel A. D. The substrates used in panels B and C with the parS-L repeats identified.