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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Microbiol. 2016 Jun 10;101(4):625–644. doi: 10.1111/mmi.13414

Figure 6.

Figure 6

Specificity of ParB binding to parSA. Binding of RedRock ParB to parS sites of mycobacteriophages Alma, Gladiator (Glad), and Echild, as well as Gordonia phage KatherineG (KathG). Gladiator, Alma, and KatherineG, all have predicted parS-L and parS-R sites flanking the parAB genes; a parS-L site is predicted upstream of Echild parA, but the sequences downstream of Echild parB are not obviously related to parS-R. Protein concentrations are as in Figure 4, panel A. B. Binding of Alma ParB to parS sites of mycobacteriophages Alma, RedRock, Gladiator (Glad), and Echild, as well as Gordonia phage KatherineG (KathG). Protein concentrations of Alma ParB from left to right are: 0, 7.8, 23.4, 70.2, 210, 632, 1896, 5690, and 17070 nM.