Table 2.
par-mediated plasmid stabilization
| Plasmid | Reporter | par locus | Plasmid retention (%)1 |
|---|---|---|---|
| pLO87 | hsp60-mCherry | None | <1 |
| pMO01 | hsp60-mCherry | parSL-parA-parB-parSR | 822 |
| pMO02 | hsp60-mCherry | parSL- -parB-parSR | 7 |
| pMO05 | hsp60-mCherry | parSL-parA-parB | 712 |
| pMO20 | None | None | 873 |
| pMO21 | None | parSL-parA-parB-parSR | 1003 |
1
Plasmid retention determined by percentages of red (pLO87, pMO01, pMO02, pMO05) or kanamycin-resistant (pMO20, pMO21) colonies after 52 generations of unselected growth.
2
Difference in pMO01 and pMO05 retention levels is not significant (p-value 0.25).
3
Difference in pMO20 and pMO21 retention is statistically significant (p-value 0.002).