Figure 2.

Cisplatin induced apoptosis/necrosis in HL-60 cells. HL-60 cells were untreated or treated with 1, 2, or 3 µM of cisplatin for 24, 48, 72, or 96 hours. The treated and untreated cells were incubated with Annexin V and PI in annexin buffer, and the cells were analyzed using a Cellometer Vision as described in the “Methods” section. The four quadrants of each unit represent the status of the cell population. The top left quadrant represents the damaged cell population, top right quadrant represents the necrotic cell population, bottom left quadrant represents the live and healthy cell population, and bottom right quadrant represents the apoptotic cell population.