Figure 2.

FA increased cell proliferation of primary Schwann cells in vitro. Schwann cells were treated with FA (0, 25, 50 and 100 µM) and cultured for 0, 24, 48 or 72 h in Dulbecco's modified Eagle's medium containing 5% fetal bovine serum. (A) Flow cytometric identification of the purity and viability of cultured primary Schwann cells (S-100 positive for Schwann cells and 7-AAD negative for live cells). (B) Cell proliferation determined by CCK-8 assay, with the results shown as the OD at 450 nm. (C) Cell proliferation determined by ³H-thymidine uptake, with the results shown as CPM. Data in the graphs are expressed as the mean ± standard error of three separate experiments. *P<0.05, vs. control. FA, ferulic acid; 7-AAD, 7-aminoactinomycin D; CCK-8, cell counting kit-8; OD, optical density; CPM, counts per minute.