Figure 5.

MEK1/ERK1/2 signalling was activated by FA in Schwann cells treated with 100 µM FA with or without 20 µM PD98059, a chemical inhibitor of MEK1, for indicated times. Western blots of (A) p-MEK1 and (B) p-ERK1/2 protein levels in Schwann cells treated by FA for 0, 15, 30 and 60 min are shown. However, the (C) p-MEK1 and (D) p-ERK1/2 protein levels in Schwann cells induced by FA for 60 min were downregulated in the presence of PD98059. GAPDH was used as an internal control. FA, ferulic acid.