Figure 3.

DAP reduced H₂O₂-induced p38 mitogen-activated protein kinase and JNK activation. (A and B) The phosphorylation level of p38 (43 kDa), ERK (42/44 kDa) and JNK (46/54 kDa) was detected by western blotting in (A) rat pheochromocytoma PC12 cells stimulated with 200 µM H₂O₂ for various times and (B) PC12 cells pretreated with DAP (5, 10, 20 µM) for 2 h, then stimulated with H₂O₂ (200 µΜ) for 30 min. Equal protein loading was confirmed by GAPDH (37 kDa) and β-actin (45 kDa). H₂O₂, hydrogen peroxide; DAP, daphnetin; JNK, c-Jun N-terminal kinases; ERK, extracellular signal-regulated kinase; p-phospho; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.