Figure 4.

Histological changes in the flaps from the three groups. Tissue sections were stained with hematoxylin and eosin, and observed microscopically (magnification, ×200) to detect histological changes. In these images, 90% of the tissue showed degeneration and necrosis of muscle fibers. The number of vessels in group I was less than that in groups II and III. The neutrophil infiltration in group I was greater than that in groups II and III. Group I, control group that was injected with 10 mg/kg saline once per day; group II, experimental group that was injected with 10 mg/kg diammonium glycyrrhizinate (DG) once per day; group III, experimental group that was injected with 10 mg/kg DG twice per day.