Gel electrophoresis pattern of high-risk HPV types using specific primer amplification. Lane M, DNA ladder (100 bp); lane 1, DNA HPV type 45 (PC; 475 bp); lane 2, positive sample DNA HPV type 45 (475 bp); lane 3, positive sample DNA HPV type 16 (325 bp); lanes 4 and 5, negative control; lane 6, DNA HPV type 18 (PC; 425 bp); lane 7, positive sample DNA HPV type 18 (425 bp). HPV, human papillomavirus; PC, positive control.