Table II.
Gene | rs NCBI | Primer sequence | Probe sequence |
---|---|---|---|
RYR3 | rs1044129 | F: ATGGAGTAATGCTTTATGGTC | S1: TTTAGGTGAATCTCCTCAAATACAA |
(A/G) | R: CAGTCACAGAGTGGTTGTAGA | S2: TTTTTTAGGTGAATCTCCTCAAATACAG | |
S3: TGAAGTGCCCACTGCAATAAAGTAA | |||
KIAA0423 | rs1053667 | F: CATGAAATCTGAGTCACATGG | S1: TTTTAGTAATCATGTTTTAATGTAGAACC |
(C/T) | R: GCTGAGAAATGAGACATACCA | S2: TTTTTTTAGTAATCATGTTTTAATGTAGAA | |
S3: TCAAACAGGATGGAACATCAGTGGATTT | |||
C14orf101 | rs4901706 | F: AAACTAAGTCATCTCCCAGATA | S1: TTTTTTTTTTAATGGGGTATTCAGTGACTAAGA |
(A/G) | R: GTCATCTGGTGAAAGACTGGA | S2: TTTTTTTTTTTTTAATGGGGTATTCAGTGACTAAGG | |
S3: TCTGCTATTTATGCAAAATTCTGTTTTTTTT | |||
GOLGA7 | rs11337 | F: CGCTGTATTTGGGAGAGAGTT | S1: TTTTTTTTTTTTACCATTAAAAGTTTCACTGTCAGAG |
(G/T) | R: CAGGCTGTAAAGTAACAAATGAG | S2: TTTTTTTTTTTTTTTACCATTAAAAGTTTCACTGTCAGAT | |
S3: ATATTGTAGGTGCTAATACTGGATTTTTTTTTTT | |||
KRT81 | rs3660 | F: GTTAGGCACCCCAACTCAAGT | S1: TTTTTATTTTTGAGAAAAGTCCTGCTCAC |
(G/C) | R: GCCAGCGGACTTCTTTCTAGG | S2: TTTTTTTTATTTTTGAGAAAAGTCCTGCTCAT | |
S3: TTGCACTATTCTATAGAAACTACAATTT |
F represents the forward primer. R represents reverse primer. S1 and S2 represent probes that match to different alleles of the SNP. S3 represents probes downstream of the SNP. miR, microRNA; SNP, single nucleotide polymorphism; NCBI, National Center for Biotechnology Information.