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. 2016 Jul 13;12(3):1867–1872. doi: 10.3892/etm.2016.3521

Table II.

Primers and probes used for genotyping of miRNA SNPs.

Gene rs NCBI Primer sequence Probe sequence
RYR3 rs1044129 F: ATGGAGTAATGCTTTATGGTC S1: TTTAGGTGAATCTCCTCAAATACAA
(A/G) R: CAGTCACAGAGTGGTTGTAGA S2: TTTTTTAGGTGAATCTCCTCAAATACAG
S3: TGAAGTGCCCACTGCAATAAAGTAA
KIAA0423 rs1053667 F: CATGAAATCTGAGTCACATGG S1: TTTTAGTAATCATGTTTTAATGTAGAACC
(C/T) R: GCTGAGAAATGAGACATACCA S2: TTTTTTTAGTAATCATGTTTTAATGTAGAA
S3: TCAAACAGGATGGAACATCAGTGGATTT
C14orf101 rs4901706 F: AAACTAAGTCATCTCCCAGATA S1: TTTTTTTTTTAATGGGGTATTCAGTGACTAAGA
(A/G) R: GTCATCTGGTGAAAGACTGGA S2: TTTTTTTTTTTTTAATGGGGTATTCAGTGACTAAGG
S3: TCTGCTATTTATGCAAAATTCTGTTTTTTTT
GOLGA7 rs11337 F: CGCTGTATTTGGGAGAGAGTT S1: TTTTTTTTTTTTACCATTAAAAGTTTCACTGTCAGAG
(G/T) R: CAGGCTGTAAAGTAACAAATGAG S2: TTTTTTTTTTTTTTTACCATTAAAAGTTTCACTGTCAGAT
S3: ATATTGTAGGTGCTAATACTGGATTTTTTTTTTT
KRT81 rs3660 F: GTTAGGCACCCCAACTCAAGT S1: TTTTTATTTTTGAGAAAAGTCCTGCTCAC
(G/C) R: GCCAGCGGACTTCTTTCTAGG S2: TTTTTTTTATTTTTGAGAAAAGTCCTGCTCAT
S3: TTGCACTATTCTATAGAAACTACAATTT

F represents the forward primer. R represents reverse primer. S1 and S2 represent probes that match to different alleles of the SNP. S3 represents probes downstream of the SNP. miR, microRNA; SNP, single nucleotide polymorphism; NCBI, National Center for Biotechnology Information.