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. 2016 Jul 20;12(3):1811–1817. doi: 10.3892/ol.2016.4888

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Copyright: © Sousa et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Validation of the CSC model. (A) Identification of a CSC subpopulation (CD133⁺ cells) in the HPAF-II pancreatic cancer cell line and evaluation of CD133 expression in cell subpopulations sorted by flow cytometry. (a) Isotype stained cells were used as controls. (b) HPAF-II cells stained with CD133/2-phycoerythrin monoclonal antibody. (B) Enrichment of HPAF-II CD133⁺ subpopulation isolated by magnetic-activated cell sorting represented on a frequency distribution histogram. The HPAF-II CD133⁺ subpopulation exhibits 8.89% of CD133⁺ cells, while the HPAF-II CD133^low subpopulation exhibited 3.07% of CD133⁺ cells, representing an enriched and a depleted population, respectively. (C) CD133 expression in HPAF-II tumor xenografts determined by immunohistochemistry (magnification, ×400). Hemotoxylin and eosin staining was used to reveal the morphology of the tumors (magnification, ×100). CSC, cancer stem cell; CD, cluster of differentiation; H&E, hematoxylin and eosin; SSC-H, measures cell granularity or internal complexity; FH2, phycoerythrin detection; % of Max, % of maximum (normalization method).