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. 2016 Jul 8;12(3):1840–1848. doi: 10.3892/ol.2016.4827

Figure 6.

Figure 6.

miR-506 inhibited osteosarcoma by targeting AEG-1 via the Wnt/β-catenin pathway signaling. (A) Western blot analysis showed that overexpression of miR-506 and knockdown of AEG-1 decreased the expression levels of β-catenin (94 kDa), c-myc (49 kDa) and cyclin D1 (34 kDa) in human osteosarcoma MG63 cells. *P<0.05 vs. miR-control or nontransfected; #P<0.05 vs. si-control. (B and C) Relative levels of β-catenin (B) mRNA and (C) protein were significantly decreased in MG63 cells transfected with si-β-catenin. *P<0.05, **P<0.01 vs. si-control. (D and E) Blocking the Wnt/β-catenin pathway using si-β-catenin (D) inhibited MG63 cell proliferation and (E) induced apoptosis of MG63 cells. *P<0.05, **P<0.01 vs. si-control. (F-H) CGP049090, a small molecule inhibitor of Wnt/β-catenin, (F) inhibited the expression of β-catenin, c-myc and cyclin D1 in MG63 cells in a concentration-dependent manner, (G) inhibited MG63 cell proliferation in a concentration-dependent manner and (H) induced apoptosis of MG63 cells (10µM CGP049090). *P<0.05, **P<0.01, ***P<0.001 vs. control (0 µM CGP049090). miR, microRNA; AEG-1, astrocyte elevated gene-1; si, small interfering RNA.