Figure 4.

Western blots depicting diminished levels of osteoprotegerin in the osteometastatic lesions are shown. A representative blot is shown here. Triplicate samples were analyzed to obtain the quantitative intensities of the protein signals for osteoprotegerin and β-actin (the loading control). The differences in the means between the groups were statistically significant when compared by analyses of variance. #Metastatic bone lesion versus control (P <0.01); ∗metastatic bone lesion versus its corresponding primary tumor (P <0.01).