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Effect of dasatinib on the phosphorylation of c-Src, the expression of c-Myc and Notch 1, and the cleavage of Notch 1. MDA-MB-231 and MDA-MB-231-JYJ breast cancer cells were incubated for 24 h in the presence of dasatinib at the indicated concentrations. Cell lysates were subjected to Western blotting using antibodies specific to phospho-c-Src (Y416), c-Myc, Notch 1, and cleaved Notch 1.
